Novel Monoclonal Antibodies and Recombined Antibodies Against Variant SARS-CoV-2. Jiajia Xie, Chengchao Ding, Jun He, Yuqing Zhang, Shuangshuang Ni, Xiangyu Zhang, Qingqing Chen, Jing Wang, Lina Huang, Hongliang He, Wenting Li, Huan Ma, Tengchuan Jin, Siping Zhang, Yong Gao. Front Immunol. 2021; 12: 715464. IF: 7.562. 期刊论文
The mutants resulted from the ongoing SARS-CoV-2 epidemic have showed resistance to antibody neutralization and vaccine-induced immune response. The present study isolated and identified two novel SARS-CoV-2 neutralizing antibodies (nAbs) from convalescent COVID-19 patients. These two nAbs (XG81 and XG83) were then systemically compared with nine nAbs that were reconstructed by using published data, and revealed that, even though these two nAbs shared targeting epitopes on spike protein, they were different from any of the nine nAbs. Compared with XG81, XG83 exhibited a higher RBD binding affinity and neutralization potency against wild-typed pseudovirus, variant pseudoviruses with mutated spike proteins, such as D614G, E484Q, and A475V, as well as the authentic SARS-CoV-2 virus. To explore potential broadly neutralizing antibodies, heavy and light chains from all 18 nAbs (16 published nAbs, XG81 and XG83) were cross-recombined, and some of the functional antibodies were screened and studied for RBD binding affinity, and neutralizing activity against pseudovirus and the authentic SARS-CoV-2 virus. The results demonstrated that several recombined antibodies had a more potent neutralization activity against variant pseudoviruses compared with the originally paired Abs. Taken together, the novel neutralizing
antibodies identified in this study are a likely valuable addition to candidate antibody drugs for the development of clinical therapeutic agents against SARS-CoV-2 to minimize mutational escape.
上一篇：Crucial mutation of spike protein evolved to variant SARS-CoV-2 escape the neutralization of convalescent plasma and RBD-specific monoclonal antibodies. Chengchao Ding, Jun He, Xiangyu Zhang, ChengCheng Jiang, Yong Sun, Yuqing Zhang, Qingqing Chen, Hongliang He, Wenting Li, Jiajia Xie*, Zhirong Liu* Yong Gao*. Frontiers in Immunology. 2021 Aug 17;12:693775. 期刊论文. IF: 7.562. 2021 Aug 17;12:693775. 期刊论文